1980) Sakai and Kurosawa 2018 homotypic synonym, validly published Parent taxon: Sulfolobus Brock et al. Electrophoretic and kinetic analyses were performed using NAD+ and ATP as substrates. Free and Open Access to Biodiversity Data. The promoter region of the Sulfolobus solfataricus gene dps (sso2079) was amplified with the primers pCdpsLacS forward and reverse (see Table 1) and cloned upstream of the reporter gene lacS from Sulfolobus solfataricus using the restriction site BgllI and NcoI and introducing the restriction site SacII at the 50 end of the promoter. C.M. Skip to main content Accessibility help We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Some Sulfolobus species, like S. solfataricus, have become model organisms of the Crenarchaeota. The species Sulfolobus solfataricus was originally described by Zillig et al. 461-470, 10.1007/s00792-009-0231-9. Nucleotide (GenBank) : U52110 Sulfolobus solfataricus putative ribokinase and Dbh genes, complete cds, and putative ATPase gene, partial cds. Tove, A.M. Grunden. In 2018, Sakai and Kurosawa established Sulfolobus solfataricus as the basonym of Saccharolobus solfataricus (Zillig et al. Sulfolobus solfataricus is a thermoacidophilic crenarchaeote that grows optimally at pH 3.0 and 80 °C . “Small multicopy, non-integrative shuttle vectors based on the plasmid pRN1 for Sulfolobus acidocaldarius and Sulfolobus solfataricus, model organisms of the (cren-)archaea”. Sulfolobus solfataricus. George Rice, Kenneth Stedman, Jamie Snyder, Blake Wiedenheft, Debbie Willits, Susan Brumfield, Timothy McDermott, and Mark J. The purified protein catalyzes the synthesis of serine from glycine and a synthetic derivative of a fragment of the natural modified folate found in S. solfataricus. Sulfolobus shibatae virus SSV1 (Fuselloviridae) SSV1 is the best-characterized virus of … In this study, the gene encoding a thermostable β-galactosidase from Sulfolobus solfataricus was cloned and expressed in Escherichia coli. One member of this genus, Sulfolobus solfataricus, uses a wide range of reduced organic compounds, including starch and its derivative maltose, as sole carbon and energy sources (20, 43). Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4) is a thermostable archaeal enzyme and a member of the error-prone and lesion-bypass Y-family. 1980.This name became validly published when it appeared on Validation List No. J Bacteriol 186:427–437 PubMed CrossRef Google Scholar The transgalactosylation reaction caused by the β-galactosidase was applied to produce lactulose using lactose as a galactose donor and fructose as an acceptor. The exchange of the a-proton of glycine with solvent protons in the absence of the modified folate was used as the activity assay. Here, we provide evidence that this thermoprotein is a multifunctional enzyme, also showing ATPase activity. In previous studies, adaptive laboratory evolution was used to generate extremely acid resistant isolates of this organism . The S.solfataricus P2 biomass was supplied by the Centre for Extremophile Research, Porton Down, UK. Among the eight Sulfolobus species established in the literature, S. islandicus, S. solfataricus, and S. acidocaldarius are by far the best described members of the genus. Thus, most of the extrachromosomal elements of the Crenarchaeota have been described in this genus. More-over, it can be plated as single colonies and cultivated both autotrophically and heterotrophically on different complex or simple nutrients (7). Our approach is to sequence subclones of mapped cosmids, followed by sequencing directly on cosmid templates with custom primers.